![]() Based on the SNP sites, candidate genes were used to obtain the specific barcode of orchid plant species and generated the corresponding DNA QR code ID card that could be immediately recognized by electronic devices. In the combined sequences, matK + ycf1 and ndhF + ycf1 were qualified for identification at the genera and species levels, suggesting the potential roles of ndhF, ycf1, matK + ycf1 and ndhF + ycf1 as candidate barcodes for orchids. The phylogenetic analyses based on genetic distance indicated that ndhF and ycf1 sequences were competent to identification at genus and species level of orchids in a single gene. By comparing the nucleotide replacement saturation of the single or combined sequences among the 4 genes, we found that these sequences reached a saturation state and were suitable for phylogenetic relationship analysis. In this study, the DNA barcoding of 4 chloroplast genes ( matK, rbcL, ndhF and ycf1 ) were used to provide theoretical basis for species identification, germplasm conservation and innovative utilization of orchids. The accurate identification of Orchids not only contributes to the safe utilization of these plants, but also it is essential to the protection and utilization of germplasm resources. The Orchidaceae is the second largest family of flowering plants, with more than 700 genera and 20,000 species distributed nearly worldwide. Finally, we present measurements and discuss electrically conductive nanomaterials for applications in nanoelectronics.ĭNA barcoding is currently an effective and widely used tool that enables rapid and accurate identification of plant species. The viscoelasticity of DNA hydrogels is shown to dramatically increase by the use of a combination of interlocking DNA tiles and DNA/carbon nanotube crosslinkers. ![]() We also demonstrate the tuning of rheological properties of hydrogel-based composites using different types of crosslinkers and spacers. These hydrogel composites showed interesting non-linear electrical properties. We demonstrate the ability of this system to self-assemble into three-dimensional percolating networks when carbon nanotubes and gold nanoparticles are incorporated into the DNA hydrogel. ![]() In this study, we have engineered, built, and characterized a variety of pure DNA hydrogels using DNA tile-based crosslinkers and different sizes of linear DNA spacers, as well as DNA hydrogel/nanomaterial composites using DNA/nanomaterial conjugates with carbon nanotubes and gold nanoparticles as crosslinkers. Among them, DNA hydrogels are known for their simple fabrication process and their tunable properties. Molecular self-assembly of DNA has been developed as an effective construction strategy for building complex materials. ![]()
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February 2023
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